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  2. Reagents
  3. Substrates and Metabolites

Substrates and Metabolites Reagents

HUMAN’s substrates and metabolites reagents are optimized for accurate measurement of key biochemical markers, such as glucose, lactate, and uric acid. These reagents support metabolic function analysis and enzymatic testing, ensuring reliable results in clinical and research settings. With high precision and compatibility, HUMAN’s reagents streamline workflows while meeting diagnostic demands.

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  • Substrates and Metabolites

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Direct bilirubin (DBIL) is a helpful marker for hepatic and post-hepatic jaundice, whereas pre-hepatic jaundice is primarily associated with an increase of indirect bilirubin. Increased direct bilirubin concentrations are found in acute and chronic viral hepatitis, liver cirrhosis, hepatocellular carcinoma, extrahepatic cholestasis, liver transplant rejection, and congential disorders like Dubin-Johnson and Rotor syndrome.

Colorimetric test for the quantitative determination of bilirubin direct in human serum and plasma.
Calcium measurements are used in the diagnosis and treatment of parathyroid disease, a variety of bone diseases, chronic renal disease and tetany (intermittent muscular contractions or spasms).

Colorimetric test for the quantitative determination of calcium total in human serum and heparinised plasma.
Total cholesterol (CHOL) measurements are used for diagnosis and therapy control of atherosclerotic coronary vascular disease and metabolic disorders such as dyslipidemia and liver diseases. The concentration of total cholesterol in serum or plasma is influenced by different factors like age, sex, diet and physical activity.

Colorimetric enzymatic test for the quantitative determination of total cholesterol in human serum and plasma.
Creatinine (CREA) is a product of the muscle metabolism. Creatinine measurements are used for diagnosis and therapy control of renal diseases and monitoring renal dialysis. The concentration of creatinine is influenced by age, body weight, and sex. Urea/BUN and creatinine determinations are frequently performed together in the differential diagnosis of kidney function.

Colorimetric (Jaffé) and enzymatic tests for the quantitative determination of creatinine in human serum, plasma and urine. Creatinine concentration in urine is a calculation basis for other urine analytes.
Glucose (GLUC) measurements are used for diagnosis and therapy control of carbohydrate metabolism disorders. Elevated glucose values (hyperglycemia) are observed in the following discorders: diabetes mellitus, gestational diabetes and Addison's disease. Decreased glucose values (hypoglycemia) are observed in neonatal hypoglycemia, congenital enzyme defects, Reye's syndrome, hepatic dysfunction, insulinomas, insulin antibodies, alcohol ingestion, neoplasms, sepsis and chronic renal failure.
HDL cholesterol (HDL-c) is regarded as a protecting lipid component against coronary vascular disease (CHD). Measurement of HDL-c cholesterol is used in the early recognition of ateriosclerotic risk and may also be used for therapy control during lipid lowering treatment. Together with LDL cholesterol it has a high diagnostic value to estimate the individual risk for CHD.

Homogeneous enzymatic color assay for the quantitative determination of HDL cholesterol in human serum and plasma.
LDL cholesterol (LDL-c) is an independent risk factor for coronary vascular disease (CHD). Epidemiological studies have shown the importance of LDL-c levels for the identification of high risk patients. HDL cholesterol (HDL-c) is regarded a protecting lipid component against coronary vascular disease (CHD). Together with HDL cholesterol LDL cholesterol has a high diagnostic value to estimate the individual risk for CHD.

Homogeneous enzymatic color assay for the quantitative determination of LDL cholesterol in human serum and plasma.
Inorganic phosphorus (PHOS, PO3) is measured for diagnosis and therapy control of various disorders such as bone diseases, chronic kidney disease, dialysis patients, kidney stones, after thyroid surgery, diseases of the parathyroid gland, chronic alcoholism, in intensive care (parenteral nutrition, ventilated patients), suspected Vit D deficiency, muscle weakness and bone pain.

UV test for quantitative determination of inorganic phosphate in human serum.
Total protein (TP) is a major component of blood and the sum of all circulating proteins. Total protein is measured for diagnosis and therapy control of a variety of diseases involving liver, kidney or bone marrow as well as other metabolic and nutritional disorders. Hypoproteinemia may be caused by abnormal synthesis, protein malnutrition, protein malabsorption, protein loss and after infusions. Hyperproteinemia may be caused by monoclonal gammopathy, severe chronic inflammatory and autoimmune processes.

Colorimetric test for the quantitative determination of total protein in human serum and plasma.
The measurement of triglycerides (TG) is used for diagnosis of primary and secondary hyperlipoproteinemias, primary and secondary prevention of coronary vascular disease (CHD), risk marker of metabolic syndrom, LDL-c calculation using the Friedewald formula as well as control of dietary and medical lipid lowering.

Enzymatic colorimetric test for the quantitative determination of triglycerides in human serum and plasma.
Urea (UREA, BUN) measurements are used in the diagnosis, differential diagnosis, assessment and therapy control of certain renal and metabolic diseases such as acute renal failure, terminal renal disease, and metabolic status of intensive care and dialysis patients. Urea and creatinine determinations are frequently performed together in the differential diagnosis of kidney function.

Conversion factor for UREA, BUN [mg/dl]
Conc. (UREA) = 2.14 x conc. (BUN); conc. (BUN) = 0.47 x conc. (UREA)

Enzymatic colorimetric test and fully enzymatic UV test for the quantitative determination of urea in human serum, plasma and urine.
Uric acid measurements are used in the diagnosis and therapy control of numerous renal and metabolic disorders, including chronic kidney disease, kidney stones, renal failure, gout, hyperlipidemia, leukemia, psoriasis, starvation or other wasting conditions and of patients receiving cytotoxic drugs or cyclosporine therapy in transplant recipients.

Enzymatic colorimetric test with or without ascorbate oxidase for the quantitative determination of uric acid in human serum, plasma and urine.

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